|
|
Registro Completo |
Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
11/07/2019 |
Data da última atualização: |
05/03/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, S. E. M.; GIMENES, M. A.; OLIVEIRA JUNIOR, J. O. L. de; COSTA, M. F.; GOMES, M. F. C.; VALENTE, S. E. S. |
Afiliação: |
MARCOS APARECIDO GIMENES, Cenargen; JOSE OSCAR LUSTOSA DE O JUNIOR, CPAMN; M. F. COSTA, UFPI; M. F. C.GOMES, UESPI; S. E. S.VALENTE, UFPI. |
Título: |
Consequences of accelerated aging for DNA integrity and seed germination of cowpea. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, article gmr18304, 2019. |
DOI: |
10.4238/gmr18304 |
Idioma: |
Inglês |
Conteúdo: |
Deterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars. MenosDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processe... Mostrar Tudo |
Palavras-Chave: |
Orthodox seeds; Seed bank. |
Thesagro: |
Vigna Unguiculata. |
Thesaurus Nal: |
Genetic resources. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199456/1/gmr18304-consequences-accelerated-aging-dna-integrity-and-seed-germination-cowpea.pdf
|
Marc: |
LEADER 02315naa a2200241 a 4500 001 2110566 005 2020-03-05 008 2019 bl uuuu u00u1 u #d 024 7 $a10.4238/gmr18304$2DOI 100 1 $aSANTOS, S. E. M. 245 $aConsequences of accelerated aging for DNA integrity and seed germination of cowpea.$h[electronic resource] 260 $c2019 520 $aDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars. 650 $aGenetic resources 650 $aVigna Unguiculata 653 $aOrthodox seeds 653 $aSeed bank 700 1 $aGIMENES, M. A. 700 1 $aOLIVEIRA JUNIOR, J. O. L. de 700 1 $aCOSTA, M. F. 700 1 $aGOMES, M. F. C. 700 1 $aVALENTE, S. E. S. 773 $tGenetics and Molecular Research$gv. 18, n. 3, article gmr18304, 2019.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 14 | |
1. | | HEBERLE, T.; KRINGEL, D. H.; DANNEMBERG, G.; EVANGELHO, J. A.; OLIVEIRA, R. P. de; DIAS, A. R. G. Atividade antimicrobiana de óleo essencial de laranja. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida. Anais... Gramado: SBCTA Regional, 2016.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Clima Temperado. |
| |
2. | | SANTOS, F. N. dos; DUARTE, L. N.; SAMBORSKI, T.; MELLO, A. F. S.; KRINGEL, D. H.; SEVERO, J. Elaboration of food products with biofortified sweet potatoes: characterization and sensory acceptability. Revista Chilena de Nutrición, v. 48, n. 1, p. 59-66, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Hortaliças. |
| |
3. | | KRINGEL, D. H.; ANTUNES, M. D.; COLUSSI, R.; DIAS, A. R. G.; ZAVAREZE, E. R.; OLIVEIRA, R. P. de. Orange essential oil inclusion compleX with B-cyclodextrin. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SESSION 6 INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: árvore que sustenta a vida. Anais... Gramado: SBCTA Regional, 2016.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Clima Temperado. |
| |
4. | | BARANZELLI, J.; KRINGEL, D. H.; BIDUSKI, B.; ZAVAREZE, E. R.; MIRANDA, M. Z. de; DIAS, A. R. G. Efeito da germinação natural e induzida nas propriedades de pasta de farinha de trigo. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25.; CIGR SECTION VI INTERNATIONAL TECHNICAL SYMPOSIUM, 10., 2016, Gramado. Alimentação: a árvore que sustenta a vida: [anais]. [s. l.: SBCTA, 2016]. Processamentos e engenharia de alimentos, poster 1413. 6 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Trigo. |
| |
5. | | EL HALAL, S. L. M.; KRINGEL, D.; MIRANDA, M. Z. de; DIAS, A. R. G.; ZAVAREZE, E. da R. Soy lecithin as an improver during freeze bread storage. In: INTERNATIONAL GLUTEN WORKSHOP, 13., 2018, Mexico. Book of abstracts... Mexico, DC: CIMMYT, 2018. p. 65.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Trigo. |
| |
6. | | EL HALAL, S. L. M.; KRINGEL, D.; MIRANDA, M. Z. de; DIAS, A. R. G.; ZAVAREZE, E. da R. Soy lecithin as an improver during freeze bread storage. In: INTERNATIONAL GLUTEN WORKSHOP, 13., 2018, Mexico City, Mexico. Proceedings... Córdoba: UCO Press Editorial Universidad de Córdoba, 2019. p. 94-96. LACC/IGW.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Trigo. |
| |
7. | | BARANZELLI, J.; HALAL, S. L. M.; KRINGEL, D. H.; PINTO, V. Z.; PAIVA, F. F.; ZAVAREZE, E.; MIRANDA, M. Z. de; DIAS, A. R. G. Atividade enzimática de trigo germinado em diferentes tempos e umidades. In: SIMPOSIO DE ALIMENTOS, 9., 2015, Passo Fundo. A indústria de alimentos e a saúde do consumidor: [anais]. Passo Fundo: UPF, 2015. Ciência de alimentos, trabalho C41. 6 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Trigo. |
| |
8. | | KRINGEL, D. H.; ANTUNES, M. D.; KLEIN, F.; CRIZEL, R. L.; WAGNER, R.; OLIVEIRA, R. P. de; DIAS, A. R. G.; ZAVAREZE, E. da R. Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/b-Cyclodextrin Inclusion Complex. Journal of Food Science, v. 82, n. 11, p. 2598-2605, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Clima Temperado. |
| |
9. | | BARANZELLI, J.; KRINGEL, D. H.; COLUSSI, R.; PAIVA, F. F.; ARANHA, B. C.; MIRANDA, M. Z. de; ZAVAREZE, E. da R.; DIAS, A. R. G. Changes in enzymatic activity, technological quality and gamma-aminobutyric acid (GABA) content of wheat flour as affected by germination. LWT. Food Science and Technology, v. 90, p. 483-490, Apr. 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Trigo. |
| |
10. | | KRINGEL, D. H.; BIDUSKI, B.; SILVA, W. M. F. da; LIM, LOONG-TAK; DIAS, A. R. G.; ZAVAREZE, E. da R. Electrospinning vs. electrospraying: a comparative assessment of the two techniques for orange essential oil encapsulation. Brazilian Journal of Food Research, Campo Mourão, v. 11 n. 2, p. 104-123, abr./jun. 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
Biblioteca(s): Embrapa Clima Temperado. |
| |
12. | | BARANZELLI, J.; BOCK, E. B.; KRINGEL, D. H.; ZAVEREZE, E. da R.; DIAS, A. R. G.; PAIVA, F. F.; TATSCH, P. O.; MIRANDA, M. Z. de. Efeito da germinação de trigo natural e induzida sobre parâmetros alveográficos e número de queda. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 10., 2016, Londrina. Anais... Londrina: Comissão Brasileira de Pesquisa de Trigo e Triticale, 2016. 5 p. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Trigo. |
| |
13. | | KRINGEL, D. H.; LANG, G. H.; DIAS, A. R. G.; GANDRA, E. A.; GANDRA, T. K. V.; ZAVAREZE, E. da R. Impact of encapsulated orange essential oil with -cyclodextrin on technological, digestibility, sensory properties of wheat cakes as well as Aspergillus flavus spoilage. Journal of the Science of Food and Agriculture, v.13, p. 5599-5607, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Clima Temperado. |
| |
14. | | BARANZELLI, J.; KRINGEL, D. H.; MALLMANN, J. F.; BOCK, E.; EL HALAL, S. L. M.; PRIETTO, L.; ZAVAREZE, E. da R.; MIRANDA, M. Z. de; DIAS, A. R. G. Impact of wheat (Triticum aestivum L.) germination process on starch properties for application in films. Starch, v. 71, n. 7, 1800262, 2019. 8 p.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Trigo. |
| |
Registros recuperados : 14 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|